Hycult Biotech is a manufacturer of research reagents in the field of innate immunity. Our products are co-developed with researchers around the globe. By connecting science with disease, these products offer innovative research solutions that enable better treatment, monitoring and prevention.

Our focus on innate immunity means we pay special attention to products relating to antimicrobial peptides, complement, acute phase proteins, LPS and microbial toxins.

Hycult Biotech further has a specific interest in inflammation and cell - and tissue damage. We contribute to these areas with our extensive knowledge in the development and production of assays, antibodies and proteins.

We believe a better treatment starts with an enhanced disease understanding and we are devoted to offer research – and diagnostic tools that assist in the translation of these insights.

At our headquarters in Uden, we, as a privately-owned company, undertake the development, manufacturing, and storage of our products. Our offerings are accessible via our Dutch headquarters, sales office in Wayne (PA) USA, and through our network of over 65 distributors present in over 50 countries.

Since 2008, we have maintained our EN ISO 13485:2016 certification, a testament to our commitment to meeting the highest standards in the industry.

EN ISO 13485:2016

- Creating assays, antibodies and proteins since 1994
- Facilities measuring 1,400 m2 (15,000 ft2) located in Uden, the Netherlands
- Laboratories, clean room and warehousing, enabling large scale production
- Hycult Biotech is ISO9001 and ISO13485 certified
- Antibody production and reagent manufacturing
- Manufacturing of assays and kit components
- Testing, development and optimization

Immunoassays, functional studies, Western blot, Flow cytometry, Immunohistochemistry, Immunoprecipitation, Immunofluorescence

Proteins, peptides, TLR/LPS ligands, isotype controls, CpG DNA

As an antibody manufacturer, Hycult Biotech has over two decades of experience in handling bioreagents. Ranging from cultivation to purification and labeling, we ensure optimal quality of your materials based on your (upscaling) needs.
- Production of monoclonal antibodies and recombinant proteins
- Culturing and downstream processing
- Lyophilization
- Custom conjugation of antibodies and proteins
- Aseptic liquid filling and vial labeling

Besides producing antibodies, Hycult Biotech also has profound experience in manufacturing and developing ELISA assays. Our dedication on aspects such as matrix validation, robustness evaluation and process verification (IQ/OQ/PQ) ensure a reliable and reproducible end product.
- Microplate coating and pouching
- Lyophilization of standards, controls and conjugates
- Production of buffers, diluents and substrates
- Filling, labelling and packaging of kit components

We also offer supporting services:
- ELISA development and optimization
- QC-testing: Isotyping, purity and application testing (FC, FS, IA, IHC, PAGE, WB)
- ELISA QC-testing: variation, stability, linearity, recovery and batch control
- Endotoxin and mycoplasma testing
- Testing and quantification of samples

Immunoassays, functional studies, Western blot, Flow cytometry, Immunohistochemistry, Immunofluorescence, immunoprecipitation

Antibody production and reagent manufacturing
- Production of monoclonal antibodies and recombinant proteins
- Culturing and downstream processing
- Lyophilization
- Custom conjugation of antibodies and proteins
- Aseptic liquid filling and vial labeling

Manufacturing of assays and kit components
- Microplate coating and pouching
- Lyophilization of standards, controls and conjugates
- Production of buffers, diluents and substrates
- Filling, labelling and packaging of kit components

Supporting services
- ELISA development and optimization
- QC-testing: Isotyping, purity and application testing (FC, FS, IA, IHC, PAGE, WB)
- ELISA QC-testing: variation, stability, linearity, recovery and batch control
- Endotoxin and mycoplasma testing
- Testing and quantification of samples

1994